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(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes

Sunday, December 1, 2019
Author/s: 
Maria V Neguembor
,Ruben Sebastian-Perez
,Francesco Aulicino
,Pablo A Gomez-Garcia
,Maria P Cosma
,Melike Lakadamyali
Year of publication: 
2018
Journal: 
Nucleic Acids Research
Link to paper: 
https://academic.oup.com/nar/article/46/5/e30/4774272
Super Resolution Data repository (click to request access to raw data): 
Figure 2: "Super resolution images (STORM) of fixed HeLa and HeLa 1.3 cells transfected with dCas9–24xSTAC and sgRNA Telomere, immunolabeled with AlexaFluor647 anti-GFP nanobody (NB)”
HeLa
HeLa 1.3
Figure S4: "Super resolution (STORM) images for HeLa cells transfected with dCas9-ˇ24xSTAC and sgRNA MUC4, immunolabeled with AlexaFluor647 anti-GFP nanobody (NB)”